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Molecular cloning of hormone-responsive genes from the yeast Saccharomyces cerevisiae.

A method for identifying yeast genes whose transcription is differentially regulated was developed. The technique is based on incorporation of the analog 4-thiouridine into nascent RNAs, which allows their purification. The purified RNAs are used to prepare cDNA copies for screening of genomic DNA l...

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Autors principals: Stetler, G L, Thorner, J
Format: Artigo
Idioma:Inglês
Publicat: 1984
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC344782/
https://ncbi.nlm.nih.gov/pubmed/6366798
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