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Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy
We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-n...
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| Autores principales: | , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Optical Society of America
2012
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3447541/ https://ncbi.nlm.nih.gov/pubmed/23024893 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.3.001972 |
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