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Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy

We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-n...

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Autori principali: Wang, Ke, Liu, Tzu-Ming, Wu, Juwell, Horton, Nicholas G., Lin, Charles P., Xu, Chris
Natura: Artigo
Lingua:Inglês
Pubblicazione: Optical Society of America 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3447541/
https://ncbi.nlm.nih.gov/pubmed/23024893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.3.001972
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