Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector.
We have developed a new recombinant DNA cloning system to isolate directly the functional unit of the human thymidine kinase (TK) gene. The system utilizes a cosmid vector that can shuttle cloned DNA sequences between bacteria and mammalian cells. A complete human cosmid library was constructed and...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1984
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6320187/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.2.414 |
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