Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector.

We have developed a new recombinant DNA cloning system to isolate directly the functional unit of the human thymidine kinase (TK) gene. The system utilizes a cosmid vector that can shuttle cloned DNA sequences between bacteria and mammalian cells. A complete human cosmid library was constructed and...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Lau, Y F, Kan, Y W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1984
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344687/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6320187/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.2.414
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