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Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA.

A rapid and convenient method has been devised to determine the binding sites for proteins on RNA. The procedure is an adaptation of one used to map DNA-protein complexes by protection against nuclease digestion. The method uses the cytotoxic ribonuclease alpha-sarcin, which hydrolyzes purines in bo...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Proc Natl Acad Sci U S A
Päätekijät: Huber, P W, Wool, I G
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: National Academy of Sciences 1984
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344668/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6364140/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.2.322
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