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Standardization of a method to detect bovine sperm-bound anti-sperm antibodies by flow cytometry

The objectives were to standardize some methodological and analytical aspects of a direct technique to detect sperm-bound anti-sperm antibodies (ASAs) in bovine semen using flow cytometry, including the effects of pre-fixation of sperm membranes with formalin buffer solution and inclusion of dead ce...

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Dettagli Bibliografici
Autori principali: Sardoy, MC, Anderson, DE, George, A, Wilkerson, ME, Skinner, S, Ferrer, MS
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3444527/
https://ncbi.nlm.nih.gov/pubmed/22925638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.theriogenology.2012.06.026
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