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Standardization of a method to detect bovine sperm-bound anti-sperm antibodies by flow cytometry
The objectives were to standardize some methodological and analytical aspects of a direct technique to detect sperm-bound anti-sperm antibodies (ASAs) in bovine semen using flow cytometry, including the effects of pre-fixation of sperm membranes with formalin buffer solution and inclusion of dead ce...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3444527/ https://ncbi.nlm.nih.gov/pubmed/22925638 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.theriogenology.2012.06.026 |
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