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Evolution of a Pathway to Novel Long-Chain Carotenoids

Using methods of laboratory evolution to force the C(30) carotenoid synthase CrtM to function as a C(40) synthase, followed by further mutagenesis at functionally important amino acid residues, we have discovered that synthase specificity is controlled at the second (rearrangement) step of the two-s...

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Detalhes bibliográficos
Main Authors: Umeno, Daisuke, Arnold, Frances H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC344396/
https://ncbi.nlm.nih.gov/pubmed/14973014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.5.1531-1536.2004
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