Generation of a human embryonic stem cell line stably expressing high levels of the fluorescent protein mCherry

AIM: The generation and characterization of a human embryonic stem cell (hESC) line stably expressing red fluorescent mCherry protein. METHODS: Lentiviral transduction of a ubiquitously-expressed human EF-1α promoter driven mCherry transgene was performed in MEL2 hESC. Red fluore-scence was assessed...

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Detalhes bibliográficos
Main Authors: Ovchinnikov, Dmitry A, Turner, Jennifer P, Titmarsh, Drew M, Thakar, Nilay Y, Sin, Dong Choon, Cooper-White, Justin J, Wolvetang, Ernst J
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Co., Limited 2012
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3443714/
https://ncbi.nlm.nih.gov/pubmed/22993664
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4252/wjsc.v4.i7.71
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