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Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosides

A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide dialdehydes generated by treatment of polyribonucleotides with alkaline phosphatase and excess periodate at pH 8 (borate buffer; no primary amine present i...

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Udgivet i:Nucleic Acids Res
Principais autores: Randerath, K., Randerath, E., Chia, L.S.Y., Gupta, R.C., Sivarajan, M.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1974
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Articles
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344335/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4375807/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/1.9.1121
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