Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosides

A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide dialdehydes generated by treatment of polyribonucleotides with alkaline phosphatase and excess periodate at pH 8 (borate buffer; no primary amine present i...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Randerath, K., Randerath, E., Chia, L.S.Y., Gupta, R.C., Sivarajan, M.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1974
Assuntos:	Articles
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344335/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4375807/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/1.9.1121
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