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Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins

Mass spectrometry (MS)-based quantification of highly homologous proteins in complex samples has proven difficult due to subtle sequence variations and the wide dynamic range of protein isoforms present. Herein, we report the use of reductive dimethylation on intact proteins to quantitatively compar...

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Autori principali: She, Yi-Min, Rosu-Myles, Michael, Walrond, Lisa, Cyr, Terry D
Natura: Artigo
Lingua:Inglês
Pubblicazione: WILEY-VCH Verlag 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3440571/
https://ncbi.nlm.nih.gov/pubmed/22144135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201100308
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