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Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins

Mass spectrometry (MS)-based quantification of highly homologous proteins in complex samples has proven difficult due to subtle sequence variations and the wide dynamic range of protein isoforms present. Herein, we report the use of reductive dimethylation on intact proteins to quantitatively compar...

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Détails bibliographiques
Auteurs principaux: She, Yi-Min, Rosu-Myles, Michael, Walrond, Lisa, Cyr, Terry D
Format: Artigo
Langue:Inglês
Publié: WILEY-VCH Verlag 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3440571/
https://ncbi.nlm.nih.gov/pubmed/22144135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201100308
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