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The synthesis of the internucleotide (phosphodiester) bond by a base-catalysed reaction.
Potassium tert-butoxide in hexamethyl phosphoramide and dimethyl formamide provides an excellent catalyst for the reaction of a 3'-hydroxyl or a 5'-hydroxyl group of a nucleoside with an appropriate nucleoside phosphorofluoridate to yield the dinucleoside phosphate. This paper describes th...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1975
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343540/ https://ncbi.nlm.nih.gov/pubmed/171626 |
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