Cargando...

`Single addition' and `transnucleotidation' reactions catalyzed by polynucleotide phosphorylase. Effect of enzymatic removal of inorganic phosphate during reaction

The reaction of the tetranucleotide, pA-A(2)-A, with 2′(3′)-0-(α-methoxyethyl)uridine 5′-diphosphate, Mg(2+) ions, and M. luteus polynucleotide phosphorylase followed by mild acid treatment to remove the blocking groups results in a 49% yield of the desired single addition product, pA-A(3)-U, togeth...

Descrición completa

Gardado en:
Detalles Bibliográficos
Main Authors: Sninsky, J.J., Bennett, G.N., Gilham, P.T.
Formato: Artigo
Idioma:Inglês
Publicado: 1974
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC343446/
https://ncbi.nlm.nih.gov/pubmed/4281080
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!