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`Single addition' and `transnucleotidation' reactions catalyzed by polynucleotide phosphorylase. Effect of enzymatic removal of inorganic phosphate during reaction
The reaction of the tetranucleotide, pA-A(2)-A, with 2′(3′)-0-(α-methoxyethyl)uridine 5′-diphosphate, Mg(2+) ions, and M. luteus polynucleotide phosphorylase followed by mild acid treatment to remove the blocking groups results in a 49% yield of the desired single addition product, pA-A(3)-U, togeth...
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| Main Authors: | , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1974
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343446/ https://ncbi.nlm.nih.gov/pubmed/4281080 |
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