Nucleotide sequences of the 5′ termini of φX174 mRNAs synthesized in vitro

When using φX174 RFI DNA as a template, in vitro, E. coli RNA polymerase synthesizes four major purine triphosphate-containing 5′ end sequences. RNase A digests of α(32)P labeled RNA were further digested with spleen exonuclease to remove the bulk of the oligonucleotides with 5′ hydroxyls and then c...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Smith, Lloyd H., Grohmann, Karel, Sinsheimer, R.L.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1974
Soggetti:	Articles
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343428/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10793707/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/1.11.1521
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