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Nucleotide sequences of the 5′ termini of φX174 mRNAs synthesized in vitro

When using φX174 RFI DNA as a template, in vitro, E. coli RNA polymerase synthesizes four major purine triphosphate-containing 5′ end sequences. RNase A digests of α(32)P labeled RNA were further digested with spleen exonuclease to remove the bulk of the oligonucleotides with 5′ hydroxyls and then c...

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Autores principales: Smith, Lloyd H., Grohmann, Karel, Sinsheimer, R.L.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1974
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC343428/
https://ncbi.nlm.nih.gov/pubmed/10793707
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