Efficient derivation of neural precursor cells, spinal motor neurons and midbrain dopaminergic neurons from human ES cells at 3% oxygen

This protocol has been designed to generate neural precursor cells (NPCs) from human embryonic stem cells (hESCs) using a physiological oxygen (O(2)) level of 3% and chemically defined conditions. The first stage involves suspension culture of hESC colonies at 3% O(2), where they acquire a neuroepit...

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Hauptverfasser: Stacpoole, SRL, Bilican, B, Webber, DJ, Luzhynskaya, A, He, XL, Compston, A, Karadottir, R, Franklin, RJM, Chandran, S
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3433269/
https://ncbi.nlm.nih.gov/pubmed/21799491
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.380
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