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Crystallization and preliminary X-ray crystallographic analysis of subunit F (F(1–94)), an essential coupling subunit of the eukaryotic V(1)V(O)-ATPase from Saccharomyces cerevisiae

V-ATPases are very complex multi-subunit enzymes which function as proton-pumping rotary nanomotors. The rotary and coupling subunit F (F(1–94)) was crystallized by the hanging-drop vapour-diffusion method. The native crystals diffracted to a resolution of 2.64 Å and belonged to space group C222(1),...

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Detalhes bibliográficos
Main Authors: Basak, Sandip, Balakrishna, Asha Manikkoth, Manimekalai, Malathy Sony Subramanian, Grüber, Gerhard
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3433196/
https://ncbi.nlm.nih.gov/pubmed/22949193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112032526
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