The rapid, simple and improved preparation of high specific activity alpha-[32P]dATP and alpha-[32P]ATP.
An improved method is described for the rapid and simple preparation of alpha-[32P]dATP and alpha-[32P]ATP from 32Pi in good yields and with specific activities from 20 - 150 Ci/mmol. The two-step procedure involves the chemical synthesis of the mononucleotide followed by its enzymic conversion to t...
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| Published in: | Nucleic Acids Res |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1977
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343245/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/600797/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.12.4347 |
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