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The rapid, simple and improved preparation of high specific activity alpha-[32P]dATP and alpha-[32P]ATP.

An improved method is described for the rapid and simple preparation of alpha-[32P]dATP and alpha-[32P]ATP from 32Pi in good yields and with specific activities from 20 - 150 Ci/mmol. The two-step procedure involves the chemical synthesis of the mononucleotide followed by its enzymic conversion to t...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Autor principal: Symons, R H
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1977
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343245/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/600797/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.12.4347
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