End labeling of enzymatically decapped mRNA.
A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1977
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343232/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/202926/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.12.4165 |
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