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End labeling of enzymatically decapped mRNA.
A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is lab...
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| Auteurs principaux: | , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1977
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343232/ https://ncbi.nlm.nih.gov/pubmed/202926 |
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