End labeling of enzymatically decapped mRNA.

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is lab...

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Bibliografiske detaljer
Main Authors: Efstratiadis, A, Vournakis, J N, Donis-Keller, H, Chaconas, G, Dougall, D K, Kafatos, F C
Format: Artigo
Sprog:Inglês
Udgivet: 1977
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC343232/
https://ncbi.nlm.nih.gov/pubmed/202926
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