The use of nuclease P1 in sequence analysis of end group labeled RNA.
A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial digestion...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1977
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343228/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/202925/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.12.4091 |
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