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The use of nuclease P1 in sequence analysis of end group labeled RNA.

A method is described for the direct sequence analysis of 20-25 nucleotides from the termini of 5'- or 3'-end-group [32P] labeled RNA. The method involves partial endonucleolytic digestion of the labeled RNA with nuclease P1 (from Penicillium citrinum) followed by separation of the partial digestion...

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Publicat a:Nucleic Acids Res
Autors principals: Silberklang, M, Gillum, A M, RajBhandary, U L
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1977
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343228/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/202925/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.12.4091
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