Overexpression of the rhodanese PspE, a single cysteine-containing protein, restores disulfide bond formation to an Escherichia coli strain lacking DsbA

Escherichia coli uses the DsbA/DsbB system for introducing disulfide bonds into proteins in the cell envelope. Deleting either dsbA or dsbB or both reduces disulfide bond formation but does not entirely eliminate it. Whether such background disulfide bond forming activity is enzyme-catalyzed is not...

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Hauptverfasser: Chng, Shu-Sin, Dutton, Rachel J., Denoncin, Katleen, Vertommen, Didier, Collet, Jean-Francois, Kadokura, Hiroshi, Beckwith, Jonathan
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3429705/
https://ncbi.nlm.nih.gov/pubmed/22809289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2958.2012.08157.x
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