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Modification of end phosphate gruops in mono- and oligonucleotides.
A method is described for selective activation of phosphomonoester end groups of oligonucleotides and nucleosidedi-(tri) phosphates via mixed anhydrides with mesitoic acid. Mixed anhydrides are synthesized in high yield and isolated by paper or DEAE-cellulose column chromatography. The ability of su...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1976
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342954/ https://ncbi.nlm.nih.gov/pubmed/1272808 |
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