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Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.
Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation s...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1976
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342908/ https://ncbi.nlm.nih.gov/pubmed/1257052 |
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