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Rapid and direct microRNA quantification by an enzymatic luminescence assay

A quantitative bioluminescence assay for rapid and sensitive microRNA (miRNA) expression analysis was developed. The assay utilizes miRNA directly as a primer for binding to a circular single-stranded DNA template, followed by rolling circle amplification. The detection of inorganic pyrophosphate (P...

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Bibliografske podrobnosti
Main Authors: Sun, Ye, Gregory, Kalvin J., Chen, Nelson G., Golovlev, Val
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3428512/
https://ncbi.nlm.nih.gov/pubmed/22759775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.06.021
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