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Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light.
We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histone...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1978
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342747/ https://ncbi.nlm.nih.gov/pubmed/724514 |
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