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Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light.

We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histone...

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Bibliografiske detaljer
Main Authors: Kunkel, G R, Martinson, H G
Format: Artigo
Sprog:Inglês
Udgivet: 1978
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC342747/
https://ncbi.nlm.nih.gov/pubmed/724514
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