Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light.
We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histone...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1978
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342747/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/724514/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.11.4263 |
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