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Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.

Micrococcal nuclease was used to eliminate endogenous protein synthesis in extracts prepared from L cells. The nuclease can be inhibited subsequently with 2'-deoxythymidine-3', 5'-diphosphate. Nuclease-treated extracts primed with exogenous reovirus mRNA, synthesized full length polyp...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Skup, D, Millward, S
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1977
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342674/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/928069/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.10.3581
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