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Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.
Micrococcal nuclease was used to eliminate endogenous protein synthesis in extracts prepared from L cells. The nuclease can be inhibited subsequently with 2'-deoxythymidine-3', 5'-diphosphate. Nuclease-treated extracts primed with exogenous reovirus mRNA, synthesized full length polyp...
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| Główni autorzy: | , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
1977
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| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342674/ https://ncbi.nlm.nih.gov/pubmed/928069 |
| Etykiety: |
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