Bacteriophage T4 RNA ligase: preparation of a physically homogeneous, nuclease-free enzyme from hyperproducing infected cells.

Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells infected by the double mutant, RNA ligase was purified to homogeneity with a 20% yield. Unlike previou...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Higgins, N P, Geballe, A P, Snopek, T J, Sugino, A, Cozzarelli, N R
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1977
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342642/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/333400/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.9.3175
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