Bacteriophage T4 RNA ligase: preparation of a physically homogeneous, nuclease-free enzyme from hyperproducing infected cells.

Infection of Escherichia coli by a bacteriophage T4 regA, gene 44 double mutant leads to about a 7-fold increase in the amount of RNA ligase obtained after infection by wild-type phage. Using cells infected by the double mutant, RNA ligase was purified to homogeneity with a 20% yield. Unlike previou...

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Bibliografiske detaljer
Main Authors:	Higgins, N P, Geballe, A P, Snopek, T J, Sugino, A, Cozzarelli, N R
Format:	Artigo
Sprog:	Inglês
Udgivet:	1977
Online adgang:	https://ncbi.nlm.nih.gov/pmc/articles/PMC342642/ https://ncbi.nlm.nih.gov/pubmed/333400
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Bacteriophage T4 RNA ligase: preparation of a physically homogeneous, nuclease-free enzyme from hyperproducing infected cells.

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