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A rapid cytosine-specific modification of E. coli tRNA Leu 1 by semicarbazide-bisulfite, a probe for polynucleotide conformations.

Cytosine residues in 32P-labeled E. coli tRNA Leu 1 were modified by treatment of the tRNA with the semicarbazide-bisulfite reagents [Hayatsu, H. (1976) Biochemistry 15, 2677-2682]. Analysis of the modification sites showed that only four cytidine residues, i.e. C35, C53, C85 and C86, reacted. They...

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Detalhes bibliográficos
Main Authors: Negishi, K, Harada, F, Nishimura, S, Hayatsu, H
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC342566/
https://ncbi.nlm.nih.gov/pubmed/409997
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