Relaxed circular SV40 DNA as cleavage intermediate of two restriction endonucleases.

We have determined the mode of cleavage of superhelical SV40 DNA (Form I) by restriction endonucleases EcoRI and HpaII at 37 degrees C. By analysis with agarose gel electrophoresis and direct examination with dark field electron microscopy, we found that a large amount of the single-nicked circular...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Ruben, G, Spielman, P, Tu, C D, Jay, E, Siegel, B, Wu, R
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1977
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342523/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/197493/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.6.1803
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