Relaxed circular SV40 DNA as cleavage intermediate of two restriction endonucleases.

We have determined the mode of cleavage of superhelical SV40 DNA (Form I) by restriction endonucleases EcoRI and HpaII at 37 degrees C. By analysis with agarose gel electrophoresis and direct examination with dark field electron microscopy, we found that a large amount of the single-nicked circular...

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Bibliographic Details

Published in:	Nucleic Acids Res
Main Authors:	Ruben, G, Spielman, P, Tu, C D, Jay, E, Siegel, B, Wu, R
Format:	Artigo
Language:	Inglês
Published:	Oxford University Press 1977
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342523/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/197493/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.6.1803
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