Bone Marrow Cells Stained by Azide-Conjugated Alexa Fluors in the Absence of an Alkyne Label

Thymidine analog 5-ethynyl-2′-deoxyuridine (EdU) has recently been introduced as an alternative to 5-bromo-2-deoxyuridine (BrdU) for cell labeling and tracking. Incorporation of EdU into replicating DNA can be detected by azide-conjugated fluors (eg, Alexa-azide) through a Cu(i)-catalyzed click reac...

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Dettagli Bibliografici
Autori principali: Lin, Guiting, Ning, Hongxiu, Banie, Lia, Qiu, Xuefeng, Zhang, Haiyang, Lue, Tom F., Lin, Ching-Shwun
Natura: Artigo
Lingua:Inglês
Pubblicazione: Mary Ann Liebert, Inc. 2012
Soggetti:
Technical Note
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3424851/
https://ncbi.nlm.nih.gov/pubmed/22380729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/scd.2012.0092
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