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Lac repressor purification without inactivation of DNA binding activity.

A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 +/- 10%. The repressor can be stored frozen indefinitely without losi...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Nucleic Acids Res
Egile Nagusiak: Rosenberg, J M, Khallai, O B, Kopka, M L, Dickerson, R E, Riggs, A D
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 1977
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342462/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/866182/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.3.567
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