Rapid incorporation kinetics and improved fidelity of a novel class of 3′-OH unblocked reversible terminators

Recent developments of unique nucleotide probes have expanded our understanding of DNA polymerase function, providing many benefits to techniques involving next-generation sequencing (NGS) technologies. The cyclic reversible termination (CRT) method depends on efficient base-selective incorporation...

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Detalhes bibliográficos
Main Authors: Gardner, Andrew F., Wang, Jinchun, Wu, Weidong, Karouby, Jennifer, Li, Hong, Stupi, Brian P., Jack, William E., Hersh, Megan N., Metzker, Michael L.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
Assuntos:
Genomics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3424534/
https://ncbi.nlm.nih.gov/pubmed/22570423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks330
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