Equimolar addition of oligoribonucleotides with T4 RNA ligase.
T4 induced RNA ligase will join equimolar concentrations of two oligoribonucleotides, (Ap)3C and p(Up) 5, to form a single product, (Ap)3Cp(Up) 5, in high yield. The presence of the 3' phosphate on p(Up)5 prevents the oligomer from adding to itself. The pH optimum of the reaction is about 7.5, but l...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1977
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342411/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/17097/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.1.85 |
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