Isolation of specific messenger RNA by adsorption of polysomes to matrix-bound antibody.

A procedure is presented for the purification of specific mRNAs, which exploits the ability of antibodies prepared against a native protein to bind to the nascent polypeptide on the polysome. Rather than precipitating these soluble antibody-polysome complexes with anti-antibody, which can lead to no...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Schutz, G, Kieval, S, Groner, B, Sippel, A E, Kurtz, D, Feigelson, P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1977
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342410/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/866179/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.1.71
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