Isolation of specific messenger RNA by adsorption of polysomes to matrix-bound antibody.
A procedure is presented for the purification of specific mRNAs, which exploits the ability of antibodies prepared against a native protein to bind to the nascent polypeptide on the polysome. Rather than precipitating these soluble antibody-polysome complexes with anti-antibody, which can lead to no...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1977
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342410/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/866179/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/4.1.71 |
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