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Rapid mapping of transposon insertion and deletion mutations in the large Ti-plasmids of Agrobacterium tumefaciens.

A procedure is presented, that has allowed the rapid assignment of transposon Tn1 and Tn7 insertion sites in the large (130 Md) nopaline Ti-plasmid pTiC58, to specific restriction enzyme fragments. Total bacterial DNA is isolated from Agrobacterium tumefaciens strain C58 mutants that carry a transpo...

詳細記述

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書誌詳細
主要な著者: Dhaese, P, De Greve, H, Decraemer, H, Schell, J, Van Montagu, M
フォーマット: Artigo
言語:Inglês
出版事項: 1979
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC342351/
https://ncbi.nlm.nih.gov/pubmed/231764
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