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On the isolation of TI-plasmid from Agrobacterium tumefaciens.

An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presen...

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Detalhes bibliográficos
Main Authors: Ledeboer, A M, Krol, A J, Dons, J J, Spier, F, Schilperoort, R A, Zaenen, I, van Larebeke, N, Schell, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1976
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC342915/
https://ncbi.nlm.nih.gov/pubmed/1257056
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