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On the isolation of TI-plasmid from Agrobacterium tumefaciens.
An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presen...
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| Main Authors: | , , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1976
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342915/ https://ncbi.nlm.nih.gov/pubmed/1257056 |
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