On the isolation of TI-plasmid from Agrobacterium tumefaciens.

An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presen...

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Publicado en:Nucleic Acids Res
Autores principales: Ledeboer, A M, Krol, A J, Dons, J J, Spier, F, Schilperoort, R A, Zaenen, I, van Larebeke, N, Schell, J
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 1976
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342915/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1257056/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/3.2.449
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