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3'End labelling of RNA with 32P suitable for rapid gel sequencing.
A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with...
Bewaard in:
| Hoofdauteurs: | , |
|---|---|
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
1978
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| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342236/ https://ncbi.nlm.nih.gov/pubmed/360168 |
| Tags: |
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