3'End labelling of RNA with 32P suitable for rapid gel sequencing.
A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with aniline,...
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| Publicado no: | Nucleic Acids Res |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1978
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/360168/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.9.3129 |
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